Composite

Part:BBa_K3252044:Design

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Full system integration of LuxOUNgRNA-dCas9GFPmut3b


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7598
    Illegal NheI site found at 6080
    Illegal NheI site found at 7357
    Illegal NheI site found at 10524
    Illegal NheI site found at 10547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7598
    Illegal BglII site found at 4254
    Illegal BamHI site found at 9636
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 11222
    Illegal SapI site found at 853
    Illegal SapI site found at 2457


Design Notes

This part was combination two system that integrated of actuator and inverter module.


Source

This part sequence was got from this reference.

Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283

References